Metabolism - Clinical and Experimental
Volume 44, Issue 11 , Pages 1380-1383 , November 1995

Triiodothyronine treatment increases substrate cycling between pyruvate carboxylase and malic enzyme in perfused rat liver

  • Kitt Falk Petersen

      Affiliations

    • Corresponding Author InformationAddress reprint requests to Kitt Falk Petersen, MD, Yale University School of Medicine, Department of Internal Medicine, Section of Endocrinology/Metabolism, Fitkin 1, 333 Cedar St, PO Box 208020, New Haven, CT 06520-8020.
    • Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA
    • Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, USA
    • ,
  • James B. Blair

      Affiliations

    • Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA
    • Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, USA
    • ,
  • Gerald I. Shulman

      Affiliations

    • Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA
    • Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, USA

Received 23 February 1995 ,Accepted 21 April 1995.

References 

  1. Katz J, Rognstad R. Futile cycles in the metabolism of glucose. Curr Top Cell Res. 1976;10:237–289
  2. Rognstad R, Katz J. Role of pyruvate kinase in the regulation of gluconeogenesis from l-lactate. J Biol Chem. 1977;252:1831–1833
  3. Freidmann B, Goodman EH, Saunders HL, et al.  An estimation of pyruvate cycling during gluconeogenesis in the perfused rat liver. Arch Biochem Biophys. 1971;143:566–578
  4. Cohen SM, Glynn P, Shulman RG. 13C NMR study of gluconeogenesis from labeled alanine in hepatocytes from euthyroid and hyperthyroid rats. In: ed 2. Proc Natl Acad Sci USA. 78:1981;p. 60–64
  5. Rognstad R. Futile cycling in liver cells from triiodothyronine treated rats. Biochem Biophys Res Commun. 1977;78:881–888
  6. Cohen SM, Ogawa S, Shulman RG. 13C NMR studies of gluconeogenesis in rat liver cells: Utilization of labeled glycerol by cells from euthyroid and hyperthyroid rats. In: ed 2. Proc Natl Acad Sci USA. 76:1979;p. 1603–1609
  7. Petersen KF, Cline GW, Blair JB, et al.  Substrate cycling between pyruvate and oxaloacetate in awake normal and 3,3′-5-triiodothyronine—treated rats. Am J Physiol. 1994;267:E273–E277
  8. Shulman GI, Rothman DL, Smith D, et al.  Mechanism of liver glycogen repletion in vivo by nuclear magnetic resonance spectroscopy. J Clin Invest. 1985;76:1229–1236
  9. Gores GJ, Kost LJ, LaRusso NF. The isolated perfused rat liver: Conceptual and practical considerations. Hepatology. 1986;6:511–517
  10. Keppler D, Decker K. Glycogen: Determination with amyloglucosidase. In:  Bergmeyer HU editors. Methods of Enzymatic Analysis. New York, NY: Academic; 1974;p. 1127–1131
  11. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1956;224:497–509
  12. Blair JB, Cook DE, Lardy HA. Influence of glucagon on the mechanism of xylitol and dihydroxyacetone in the isolated perfused rat liver. J Biol Chem. 1973;248:3601–3607
  13. Drake RL, Parks WC, Thompson EW. Insulin stimulation of hepatic malic enzyme activity in normal and diabetic rats controlled by different regulatory processes. J Biol Chem. 1983;258:6008–6010
  14. Yeung KK, Carrico RJ. Purification of malic enzyme by affinity chromatography on immobilized N6-(6aminohexyl)-adenosine 2′,5′-bisphosphate. Anal Biochem. 1976;74:369–375
  15. Shulman GI, Rossetti L, Rothman DL, et al.  Quantitative analysis of glycogen repletion by nuclear magnetic resonance spectroscopy in the conscious rat. J Clin Invest. 1987;80:387–393
  16. Rognstad R. Control of pyruvate kinase flux during gluconeogenesis in isolated liver cells. Int J Biochem. 1976;7:403–408
  17. Schimerlik MI, Cleland WW. Inhibition and alternate-substrate studies on the mechanism of malic enzyme. Biochemistry. 1977;16:565–570

☆ Supported by grants from the US Public Health Service (DK 40936, DK 34989, DK 45735, and RR 03475) and a grant from the Juvenile Diabetes Foundation International.

PII: 0026-0495(95)90133-7

Metabolism - Clinical and Experimental
Volume 44, Issue 11 , Pages 1380-1383 , November 1995

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