Metabolism - Clinical and Experimental
Volume 44, Issue 6 , Pages 730-738 , June 1995

Glucocorticoids upregulate high-affinity, high-density lipoprotein binding sites in rat hepatocytes

  • Alexander V. Bocharov

      Affiliations

    • Corresponding Author InformationAddress reprint requests to Alexander V. Bocharov, PhD, Cardiology Research Center, Institute of Experimental Cardiology, 3rd Cherepkovskaya str. 15A, Moscow 121552, Russia.
    • Cardiology Research Center, Moscow, Russia
    • Laboratory of Hepato-Gastroenterology, Faculty of Medicine, Nice University, Nice, France
    • ,
  • Wei Huang

      Affiliations

    • Cardiology Research Center, Moscow, Russia
    • Laboratory of Hepato-Gastroenterology, Faculty of Medicine, Nice University, Nice, France
    • ,
  • Tatiana G. Vishniakova

      Affiliations

    • Cardiology Research Center, Moscow, Russia
    • Laboratory of Hepato-Gastroenterology, Faculty of Medicine, Nice University, Nice, France
    • ,
  • Elena V. Zaitseva

      Affiliations

    • Cardiology Research Center, Moscow, Russia
    • Laboratory of Hepato-Gastroenterology, Faculty of Medicine, Nice University, Nice, France
    • ,
  • Ella G. Frolova

      Affiliations

    • Cardiology Research Center, Moscow, Russia
    • Laboratory of Hepato-Gastroenterology, Faculty of Medicine, Nice University, Nice, France
    • ,
  • Patrick Rampal

      Affiliations

    • Cardiology Research Center, Moscow, Russia
    • Laboratory of Hepato-Gastroenterology, Faculty of Medicine, Nice University, Nice, France
    • ,
  • Roger Bertolotti

      Affiliations

    • Cardiology Research Center, Moscow, Russia
    • Laboratory of Hepato-Gastroenterology, Faculty of Medicine, Nice University, Nice, France

Received 12 April 1994 ,Accepted 8 September 1994.

References 

  1. Steinberg D. Lipoproteins and the pathogenesis of atherosclerosis. Circulation. 1987;76:508–514
  2. Abbott RD, Wilson PWF, Kannel WB, et al.  High density lipoproteins cholesterol screening and myocardial infarction. Arteriosclerosis. 1984;4:303–322
  3. Livishits G, Weisbort J, Meshulam N, et al.  Multivariate analysis of the twenty-year follow-up of the Donolo-Tel Aviv prospective coronary artery disease study and the usefulness of the high density lipoproteins cholesterol percentage. Am J Cardiol. 1989;63:676–681
  4. Glomset JA. The plasma lecithin:cholesterol acyltransferase reaction. J Lipid Res. 1968;9:155–167
  5. Gordon DJ, Rifkind BM. High-density lipoprotein: The clinical implication of recent studies. N Engl J Med. 1989;321:1311–1316
  6. Ettinger WH, Klinefelter HF, Kwiterovitch PO. Effect of short-term, low-dose corticosteroids on plasma lipoprotein lipids. Atherosclerosis. 1987;63:167–172
  7. Ettinger WH, Hazzard WR. Prednisone increases very low density lipoprotein and high density lipoprotein in healthy men. Metabolism. 1988;37:1055–1058
  8. Bagdade JD, Porte D, Bierman EL. Steroid-induced lipemia. Arch Intern Med. 1970;125:129–134
  9. Nashel DJ. Is atherosclerosis a complication of long-term corticosteroid treatment?. Am J Med. 1986;80:925–929
  10. Taskinen MR, Nikkila EA, Pelkonen R, et al.  Plasma lipoproteins, lipolytic enzymes, and very low density lipoprotein triglyceride in Cushing's syndrome. J Clin Endocrinol Metab. 1983;57:619–626
  11. Taskinen MR, Nikkila EA, Yki-Jarvinen H, et al.  Short-term effects of prednisone on serum lipids and high density lipoprotein subtractions in healthy men. J Clin Endocrinol Metab. 1988;67:291–299
  12. Elshourbagy NA, Boguski MS, Liao WSL, et al.  Expression of rat apolipoprotein A-IV and A-I genes: mRNA induction during development and in response to glucocorticoids and insulin. In: ed 2. Proc Natl Acad Sci USA. 82:1985;p. 8242–8246
  13. Jansen H, van Tol A, Auwerx J, et al.  Opposite regulation of hepatic lipase and lecithin:cholesterol acyltransferase by glucocorticoids in rats. Biochim Biophys Acta. 1992;1128:181–185
  14. Hylemon PB, Gurley EC, Stravitz RT, et al.  Hormonal regulation of cholesterol 7o-hydroxylase mRNA levels and transcriptional activity in primary rat hepatocyte cultures. J Biol Chem. 1992;267:16866–16871
  15. De Crom MPG, Van Haperen MJ, Puchois P, et al.  Binding characteristics of high density lipoprotein subclasses to porcine liver, adrenal and skeletal muscle plasma membranes. Int J Biochem. 1989;21:649–656
  16. Bachorik PS, Franklin FA, Virgil DG, et al.  Reversible high affinity uptake of apo E-free high density lipoproteins in cultured pig hepatocytes. Arteriosclerosis. 1985;5:142–152
  17. Schouten D, Kleinherenbrink-Stins MF, Brouwer A, et al.  Interaction in vivo and in vitro of apolipoprotein E-free high density lipoprotein with parenchymal, endothelial and Kupffer cells from rat liver. Biochem J. 1988;256:615–621
  18. Schouten D, Kleinherenbrink-Stins MF, Brouwer A, et al.  Characterization in vitro of interaction of human apolipoprotein E-free high density lipoprotein with human hepatocytes. Arteriosclerosis. 1990;10:1127–1135
  19. Bocharov AV, Huang W, Zaitseva EV, et al.  Glucocorticoid-dependent regulation of expression of HDL-binding sites in rat hepatocytes. Bull Exp Biol Med. 1994;1:47–50
  20. Zaitseva EV, Huang W, Vishnyakova TG, et al.  Characterization of HDL-binding sites in cultured rat hepatocytes, liver endothelial and Kupffer cells. Bull Exp Biol Med. 1994;3:348–354
  21. Dashti N, Wolfbauer G, Alaupovic P. Binding and degradation of human high-density lipoproteins by human hepatoma cell line HepG2. Biochim Biophys Acta. 1985;833:100–110
  22. Barbaras R, Puchois P, Fruchart J-C, et al.  Purification of an apolipoprotein A binding protein from mouse adipose cells. Biochem J. 1990;269:767–773
  23. De Crom RPG, van Haperen R, Willemsen R, et al.  High density lipoprotein-binding proteins in porcine liver. In: Isolation and histological localization. ed 2. Arterioscler Tromb. 12:1991;p. 325–331
  24. Hidaka H, Fidge N. Affinity purification of the hepatic high-density lipoprotein receptor identifies two acadic glycoproteins and enables further characterization of their properties. Biochem J. 1992;284:161–167
  25. Redgrave TG, Roberts DCK, West CE. Separation of plasma lipoproteins by density gradient centrifugation. Anal Biochem. 1975;65:42–49
  26. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–685
  27. McFarlane AS. Efficient trace-labelling of proteins with iodine. Nature. 1958;182:53–54
  28. Bilheimer PW, Eisenberg S, Levy P. The metabolism of very low density lipoproteins. I. Preliminary in vitro and in vivo observations. Biochim Biophys Acta. 1972;260:212–221
  29. Smirnova OV, Vishnyakova TG, Rozen VB. The role of some hormones with total metabolic action in the expression of sex differentiation of the liver by a specific estrogen-binding protein. Prob Endocr USSR. 1989;35:59–63
  30. Vishniakova TG, Shnyra AA, Botcharov AV, et al.  Estimation of a direct effect of estrogen on cultured hepatocytes tested by the changes in the unusual estrogen-binding protein level. Biokhimia. 1989;54:694–701
  31. Berry MN, Friend DS. High yield preparation of isolated rat liver parenchymal cells. J Cell Biol. 1969;43:506–520
  32. Seglen PO. Preparation of isolated rat liver cells. In: ed 2.  Prescott DM editors. Methods in Cell Biology. vol 13:New York, NY: Academic; 1976;p. 29–83
  33. Bradford M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248–256
  34. Sperry WM, Webb N. A revision of the Schoenheimer-Sperry method for cholesterol determination. J Biol Chem. 1950;187:97–106
  35. Ettiniger WH, Dysko RC, Clarson TB. Prednisone increases low density lipoproteins in cynomolgus monkey fed saturated fat and cholesterol. Arteriosclerosis. 1989;9:848–855
  36. Gustafsson JA, Dahlman-Wright K, Stromstedt PE, et al.  Structure-function aspects of the glucocorticoid receptor. In: ed 2. Int Symp Princess Takamatsu Cancer Res Fund. 21:1990;p. 137–155
  37. Nawa K, Nakamura T, Kumatori K, et al.  Glucocorticoid-dependent expression of the albumin gene in adult rat hepatocytes. J Biol Chem. 1986;261:16883–16888
  38. Kulkarni AB, Reinke R, Feigelson P. Acute phase mediators and glucocorticoids elevate 1-acid glycoprotein gene transcription. J Biol Chem. 1985;260:15386–15389
  39. Turcotte B, Guertin M, Chevrette M, et al.  Rat alpha 1-fetoprotein messenger RNA: 5′-End sequence and glucocorticoid suppressed liver transcription in an improved nuclear run-off assay. Nucleic Acids Res. 1985;13:2387–2398
  40. Noda C, Yakiyma M, Nakamura T, et al.  Requirements of both glucocorticoids and glucagon as co-inducers for activation of transcription of the serine dehydrogenase gene in cultured rat hepatocytes. J Biol Chem. 1988;263:1464–1468
  41. Panin LE, Trubitsina OM, Tretjakova TA, et al.  The binding of 3H-corticosterone with rat hepatocytes. Bull Exp Biol Med. 1992;10:362–364
  42. McKnight GL, Reasoner J, Gilbert T, et al.  Cloning and expression of a cellular high density lipoprotein binding site that upregulated by cholesterol loading cells. J Biol Chem. 1992;267:12131–12141
  43. Sviridov DD, Pavlov MY, Safonova IG, et al.  Inhibition of cholesterol synthesis and esterification regulates high density lipoprotein interaction with isolated epithelial cells of human intestine. Atherosclerosis. 1990;31:1821–1830
  44. Salter AM, Fisher SC, Brindley DN. Binding low density lipoprotein to monolayer cultures of rat hepatocytes is increased by insulin and decreased by dexamethasone. FEBS Lett. 1987;220:159–162
  45. Sasahary T, Kobori S, Kasho M, et al.  The metabolic fate of apolipoprotein AI—containing lipoproteins internalized into Hep G2 cells: Resecreted lipoproteins as a potent inducer for cholesterol efflux. Atherosclerosis. 1994;106:179–190

☆ Supported by the Academy of Medical Science, Russia.

PII: 0026-0495(95)90185-X

Metabolism - Clinical and Experimental
Volume 44, Issue 6 , Pages 730-738 , June 1995

Article Tools