Experimentally induced acute hyperinsulinemia stimulates endogenous nitric oxide production in humans: Detection using urinary  excretion

Tsukahara, Hirokazu; Kikuchi, Kiyoshi; Tsumura, Kumi; Kimura, Kouki; Hata, Ikue; Hiraoka, Masahiro; Sudo, Masakatsu

« Previous Next »Metabolism - Clinical and Experimental
Volume 46, Issue 4 , Pages 406-409, April 1997

Experimentally induced acute hyperinsulinemia stimulates endogenous nitric oxide production in humans: Detection using urinary excretion☆

Hirokazu Tsukahara
- Address reprint requests to Hirokazu Tsukahara, MD, Department of Pediatrics, Fukui Medical School, Fukui 910-11, Japan.
- Department of Pediatrics, Fukui Medical School, Fukui, Japan
- Department of Pediatrics, Shimane Prefectural Central Hospital, Izumo, Japan
Kiyoshi Kikuchi
- Department of Pediatrics, Fukui Medical School, Fukui, Japan
- Department of Pediatrics, Shimane Prefectural Central Hospital, Izumo, Japan
Kumi Tsumura
- Department of Pediatrics, Fukui Medical School, Fukui, Japan
- Department of Pediatrics, Shimane Prefectural Central Hospital, Izumo, Japan
Kouki Kimura
- Department of Pediatrics, Fukui Medical School, Fukui, Japan
- Department of Pediatrics, Shimane Prefectural Central Hospital, Izumo, Japan
Ikue Hata
- Department of Pediatrics, Fukui Medical School, Fukui, Japan
- Department of Pediatrics, Shimane Prefectural Central Hospital, Izumo, Japan
Masahiro Hiraoka
- Department of Pediatrics, Fukui Medical School, Fukui, Japan
- Department of Pediatrics, Shimane Prefectural Central Hospital, Izumo, Japan
Masakatsu Sudo
- Department of Pediatrics, Fukui Medical School, Fukui, Japan
- Department of Pediatrics, Shimane Prefectural Central Hospital, Izumo, Japan

Received 27 June 1996; accepted 6 September 1996.

Abstract

Insulin-mediated glucose metabolism in skeletal muscle is associated with a proportional increase in muscle perfusion. The vasodilatory effect of insulin is thought to be mediated in part by endothelium-derived nitric oxide (NO). The present study was performed to determine whether acute hyperinsulinemia has any stimulatory effect on endogenous NO production in humans. Bolus intravenous injection of insulin (0.1 IU/kg body weight) caused a significant increase in urinary excretion of together with a significant decrease in blood pressure, whereas saline infusion alone had no effect on these parameters. The increased NO response to insulin was almost comparable to that obtained with infusion of 30 g l-arginine. The acute effect of hyperinsulinemia on endogenous NO formation supports the concept that NO may mediate the vasodilatory action of insulin in humans.