Continuous glucose monitoring in the free-moving rat

Thomé-Duret, V.; Aussedat, B.; Reach, G.; Gangnerau, M.N.; Lemonnier, F.; Klein, J.C.; Zhang, Y.; Hu, Y.; Wilson, G.S.

« Previous Next »Metabolism - Clinical and Experimental
Volume 47, Issue 7 , Pages 799-803, July 1998

Continuous glucose monitoring in the free-moving rat☆

V. Thomé-Duret
- INSERM U341, Département de Diabétologie, Hôtel-Dieu, Paris, France
- École des Mines, Fontainebleau, France
- Department of Chemistry, University of Kansas, Lawrence, KS, USA
B. Aussedat
- INSERM U341, Département de Diabétologie, Hôtel-Dieu, Paris, France
- École des Mines, Fontainebleau, France
- Department of Chemistry, University of Kansas, Lawrence, KS, USA
G. Reach
- Address reprint requests to G. Reach, MD, INSERM U341, Service de Diabétologie, Hôtel-Dieu, 1, place du Parvis Notre-Dame, 75004 Paris, France.
- INSERM U341, Département de Diabétologie, Hôtel-Dieu, Paris, France
- École des Mines, Fontainebleau, France
- Department of Chemistry, University of Kansas, Lawrence, KS, USA
M.N. Gangnerau
- INSERM U341, Département de Diabétologie, Hôtel-Dieu, Paris, France
- École des Mines, Fontainebleau, France
- Department of Chemistry, University of Kansas, Lawrence, KS, USA
F. Lemonnier
- INSERM U341, Département de Diabétologie, Hôtel-Dieu, Paris, France
- École des Mines, Fontainebleau, France
- Department of Chemistry, University of Kansas, Lawrence, KS, USA
J.C. Klein
- INSERM U341, Département de Diabétologie, Hôtel-Dieu, Paris, France
- École des Mines, Fontainebleau, France
- Department of Chemistry, University of Kansas, Lawrence, KS, USA
Y. Zhang
- INSERM U341, Département de Diabétologie, Hôtel-Dieu, Paris, France
- École des Mines, Fontainebleau, France
- Department of Chemistry, University of Kansas, Lawrence, KS, USA
Y. Hu
- INSERM U341, Département de Diabétologie, Hôtel-Dieu, Paris, France
- École des Mines, Fontainebleau, France
- Department of Chemistry, University of Kansas, Lawrence, KS, USA
G.S. Wilson
- INSERM U341, Département de Diabétologie, Hôtel-Dieu, Paris, France
- École des Mines, Fontainebleau, France
- Department of Chemistry, University of Kansas, Lawrence, KS, USA

Received 31 July 1997; accepted 15 January 1998.

Abstract

The aim of this work was to set up an experimental model of glycemic fluctuations for assessing in the conscious freely moving rat, the performance of a continuous glucose-monitoring system, using a pocket-calculator—size electronic control unit and a miniaturized subcutaneous glucose sensor. The well-known triphasic glycemic pattern following streptozotocin injection (initial peak and secondary hypoglycemia preceding the establishment of permanent hyperglycemia) was used as a way to obtain spontaneous changes in blood glucose level over a wide concentration range. This report demonstrates that streptozotocin injection produced highly reproducible changes in the current generated by the sensor: an initial peak and a secondary nadir, during which blood sampling provided the evidence of hyperglycemia associated with immunoreactive hypoinsulinemia, and of hypoglycemia associated with hyperinsulinemia, respectively. This reproducible experimental model should be valuable for the assessment of a continuous glucose-monitoring system.

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☆ Supported by Institut National de la Santé et de la Recherche Médicale (grant CNAMTS), National Institutes of Health grant no. DK 30718, Aide aux Jeunes Diabétiques (grant to V.T.D.).

PII: S0026-0495(98)90115-9

« Previous Next »Metabolism - Clinical and Experimental
Volume 47, Issue 7 , Pages 799-803, July 1998

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