Effects of thyrotropin, sodium fluoride and ions on thyroid slice metabolism

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      Abstract

      Activation of adenyl cyclase with resultant formation of cyclic 3′, 5′-AMP has been implicated in the action of thyrotropin (TSH) on thyroid. Since sodium fluoride increases adenyl cyclase activity in thyroid and also stimulates thyroid slice glucose oxidation and phospholipogenesis, we compared the effects of TSH and fluoride, singly and in combination, on glucose-1-14C oxidation and 32P incorporation into phospholipid in sheep thyroid slices. Although these effects were superficially alike, and were similarly affected by changes in the ionic composition of the incubation medium, no synergism between TSH and fluoride effects was noted and stimulation induced by maximal effective concentrations of NaF was augmented by the addition of TSH. These data suggest that the sites at which these compounds interact on the thyroid to exert their effects are different. The significance of these findings in relation to the possible role of the adenyl cyclase-cyclic 3′, 5′-AMP system in controlling thyroid metabolism is discussed.
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      References

        • Pastan I.
        • Katzen R.
        Activation of adenyl cyclase in thyroid homogenates by thyroid-stimulating hormone.
        Biochem. Biophys. Res. Comm. 1967; 29: 762
        • Klainer L.M.
        • Chi Y.-M.
        • Friedberg S.C.
        • Rall T.W.
        • Sutherland E.W.
        Adenyl cyclase. IV. The effects of neurohormones on the formation of adenosine 3′, 5′-phosphate by preparations from brain and other tissues.
        J. Biol. Chem. 1962; 237: 1239
        • Gilman A.G.
        • Rall T.W.
        Studies on the relation of cyclic 3′, 5′-AMP (CA) to TSH action in beef thyroid slices.
        in: Fed. Proc.25. 1966: 617
        • Sutherland E.W.
        • Rall T.W.
        • Menon T.W.
        Adenyl cyclase. I. Distribution, preparation and properties.
        J. Biol. Chem. 1961; 237: 1220
        • Rabinowitz M.
        • Desalles L.
        • Meisler J.
        • Lorand L.
        Distribution of adenyl cyclase activity in rabbit skeletal muscle fractions.
        Biochim. Biophys. Acta. 1965; 97: 29
        • Øye I.
        • Sutherland E.W.
        The effects of epinephrine and other agents on adenyl cyclase in the cell membrane of avian erythrocytes.
        Biochim. Biophys. Acta. 1966; 127: 347
        • Pastan I.
        • Macchia V.
        • Katzen R.
        The effects of fluoride on the metabolic activity of thyroid slices.
        Endocrinology. 1968; 83: 157
        • O'Malley B.W.
        • Field J.B.
        Effects of ions and metabolic inhibitors on thyroid stimulating hormone stimulation of glucose oxidation in thyroid slices.
        Biochim. Biophys. Acta. 1964; 90: 349
        • Burke G.
        Effects of inorganic iodide on thyroid stimulation in vitro.
        Endocrinology. 1968; 82: 1170
        • Burke G.
        Effects of iodotyrosines on basal and stimulated glucose oxidation and phospholipogenesis in sheep thyroid slices.
        Endocrinology. 1968; 83: 495
        • Kögl F.
        • Van Deenen L.L.M.
        Metabolism and function of phosphatides. Investigation on the action of thyrotropic hormone on thyrophosphatide.
        Acta Endocr. (Kobenhavn). 1961; 36: 9
        • Winer B.J.
        Statistical Principles in Experimental Design. McGraw-Hill, New York1962: 298-349
        • Snedecor G.W.
        Statistical Methods. Iowa State College Press, Ames, Iowa1959: 333
        • Oka H.
        • Field J.B.
        Effect of ions on TSH stimulation of 32P incorporation into thyroid slice phospholipid.
        Amer. J. Physiol. 1966; 211: 1357
        • Burke G.
        The cell membrane: A common site of action of thyrotropin (TSH) and long-acting thyroid stimulator (LATS).
        Metabolism. 1969; 18: 720-729
        • Altman M.
        • Oka H.
        • Field J.B.
        Effect of TSH, acetylcholine, epinephrine, serotonin and synkavite on 32P incorporation into phospholipid in dog thyroid slices.
        Biochim. Biophys. Acta. 1966; 116: 586
        • Field J.B.
        • Pastan I.
        • Johnson P.
        • Herring B.
        In vitro stimulation of the hexose monophosphate pathway in thyroid by thyroid-stimulating hormone.
        Biochem. Biophys. Res. Comm. 1959; 1: 284
        • Pastan I.
        • Wollman S.H.
        Colloid droplet formation in dog thyroid in vitro. Induction by dibutyryl cyclic-AMP.
        J. Cell Biol. 1967; 35: 262
        • Kaneko T.
        • Zor U.
        • Field J.B.
        Thyroid-stimulating hormone and prostaglandin E1. Stimulation of cyclic 3′, 5′-adenosine monophosphate in thyroid slices.
        Science. 1969; 163: 1062
        • Chase L.R.
        • Fedak S.A.
        • Aurbach G.D.
        Activation of skeletal adenyl cyclase by parathyroid hormone in vitro.
        Endocrinology. 1969; 84: 761