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Lactate inhibition of lipolysis in exercising man

  • A.E. Boyd III
    Footnotes
    Affiliations
    U. S. Army Research Institute of Environmental Medicine, Natick, Mass. USA

    the Division of Endocrinology, Department of Medicine, Duke University Medical Center, Durham, N.C. USA
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  • S.R. Giamber
    Footnotes
    Affiliations
    U. S. Army Research Institute of Environmental Medicine, Natick, Mass. USA

    the Division of Endocrinology, Department of Medicine, Duke University Medical Center, Durham, N.C. USA
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  • M. Mager
    Correspondence
    Reprint requests should be addressed to Dr. M. Mager, Biochemistry and Pharmacology Laboratory, U.S. Army Research Institute of Environmental Medicine, Natick, Mass. 01760.
    Footnotes
    Affiliations
    U. S. Army Research Institute of Environmental Medicine, Natick, Mass. USA

    the Division of Endocrinology, Department of Medicine, Duke University Medical Center, Durham, N.C. USA
    Search for articles by this author
  • H.E. Lebovitz
    Footnotes
    Affiliations
    U. S. Army Research Institute of Environmental Medicine, Natick, Mass. USA

    the Division of Endocrinology, Department of Medicine, Duke University Medical Center, Durham, N.C. USA
    Search for articles by this author
  • Author Footnotes
    1 A. E. Boyd, III, M.D., MAJ, MC: Research Internist, U.S. Army Research Institute of Environmental Medicine, Natick, Mass.; presently with Division of Endocrinology, Tufts New England Medical Center, Boston, Mass.
    2 S. R. Giamber, M.D., MAJ, MC: Research Internist, U.S. Army Research Institute of Environmental Medicine, Natick, Mass.; presently with Cardiac Catheter Unit, Massachusetts General Hospital, Boston, Mass.
    3 M. Mager, Ph.D.: Director, Biochemistry and Pharmacology Laboratory, U.S. Army Research Institute of Environmental Medicine, Natick, Mass.
    4 H. E. Lebovitz, M.D.: Professor of Medicine, and Chief, Endocrine Unit, Duke University Medical Center, Durham, N.C.; supported in part by Research Grant AM 01324 and Career Development Award KAM-17-954.
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      Abstract

      The role of rising lactate levels on substrate mobilization in exercising man is unclear. To define the role of increasing lactate levels, D,L-sodium lactate, 6 meq/kg, was infused into six exercising normal males. A mild work load was chosen that increased arterial free fatty acids (FFA) and glycerol, but did not increase lactate significantly. Infused sodium bicarbonate and sodium chloride of equal volume and osmolality served as controls. The marked rise in arterial lactate and pyruvate to 8.8 ± 0.31 μM/ml and 0.328 ± 0.023 μM/ml (mean ± SEM), respectively, resulted in a significant inhibition of the increase of plasma FFA and glycerol, which occurred during the control studies. The decreased release of these constituents could not be attributed to an “insulin effect”, since the concentration of arterial insulin decreased during exercise and was similar during each infusion period. It appears that an increase in plasma lactate or pyruvate, or both, results in a direct inhibition of exercise-mediated lipolysis in man.
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      References

        • Fritz TB
        • Davis DG
        • Holtrop RH
        • Dundee H
        Fatty acid oxidation by skeletal muscle during rest and activity.
        Am J Physiol. 1958; 194: 379
        • Gordon Jr, RS
        Unesterified fatty acid in human blood plasma. II. The transport function of unesterified fatty acid.
        J Clin Invest. 1957; 36: 810
        • Carlson LA
        • Pernow B
        Studies on blood lipids during exercise. I. Arterial and venous plasma concentrations of unesterified fatty acids.
        J Lab Clin Med. 1959; 53: 833
        • Friedberg SJ
        • Harlan Jr, WR
        • Trout DL
        • Estes Jr, EH
        The effect of exercise on the concentration and turnover of plasma nonesterified fatty acids.
        J Clin Invest. 1960; 39: 215
        • Friedberg SJ
        • Estes Jr, EH
        Direct evidence for the oxidation of free fatty acids by peripheral tissues.
        J Clin Invest. 1962; 41: 677
        • Bosu A
        • Passamore R
        • Strong JA
        The effect of exercise on the level of non-esterified fatty acids in the blood.
        Q J Exp Physiol. 1960; 45: 312
        • Pruett EDR
        FFA mobilization during and after prolonged severe muscular work in men.
        J Appl Physiol. 1970; 29: 809
        • Cobb LA
        • Johnson WP
        Hemodynamic relationships of anaerobic metabolism and plasma free fatty acids during prolonged, strenuous exercise in trained and untrained subjects.
        J Clin Invest. 1963; 42: 800
        • Havel RJ
        • Pernow B
        • Jones NL
        Uptake and release of free fatty acids and other metabolites in the legs of exercising men.
        J Appl Physiol. 1967; 23: 90
        • Havel RJ
        • Naimark A
        • Borchgrevink CF
        Turnover rate and oxidation of free fatty acids of blood plasma in man during exercise: studies during continuous infusion of palmitate-1-C14.
        J Clin Invest. 1963; 42: 1054
        • Havel RJ
        • Carlson LA
        • Ekelund LG
        • Holmgren A
        Turnover rate and oxidation of different free fatty acids in man during exercise.
        J Appl Physiol. 1964; 19: 613
        • Havel RJ
        Influence of intensity and duration of exercise on supply and use of fuels.
        in: Pernow B Saltin B Muscle Metabolism During Exercise. Plenum, New York1971: 315
        • Björntorp P
        The effect of lactic acid on adipose tissue metabolism in vitro.
        Acta Med Scand. 1965; 178: 253
        • Dieterle P
        • Dieterle C
        • Bottermann P
        • Schwarzk K
        • Henner J
        In vitro versuche zur antilipolytischen wirkung von milchsaure.
        Diabetologia. 1969; 5: 238
        • Miller H
        • Issekutz Jr, B
        • Rodahl K
        Effect of exercise on the metabolism of fatty acids in the dog.
        Am J Physiol. 1963; 205: 167
        • Issekutz Jr, B
        • Miller H
        Plasma free fatty acids during exercise and the effect of lactic acid.
        in: Proc Soc Exp Biol Med. 110. 1962: 237
        • Houghton CRS
        • Hawkins R
        • Williamson D
        • Krebs HA
        An explanation for the lowering of blood ketone-body concentration in starved rats during short-term exercise.
        Biochem J. 1971; 124: 56
        • Astrand PO
        • Saltin B
        Oxygen uptake during the first minutes of heavy muscular exercise.
        J Appl Physiol. 1961; 16: 971
        • Dixon WJ
        • Massey FJ
        Introduction to Statistical Analysis. McGraw-Hill, New York1951: 103
        • Lindquist EF
        Design and Analysis of Experiments in Psychology and Education. Houghton Mifflin, Boston1953: 237
        • Winer BJ
        Statistical Principles in Experimental Design. McGraw-Hill, New York1962: 94
        • Mager M
        • Farese G
        What is “true” blood glucose?.
        Am J Clin Pathol. 1965; 44: 104
        • Farese G
        • Mager M
        Use of a rapid ultrafiltration technique in the determination of plasma glycerol.
        J Lipid Res. 1970; 11: 274
        • Dole V
        • Meinertz H
        Micro determination of long-chain fatty acids in plasma and tissues.
        J Biol Chem. 1960; 235: 2595
        • Mosinger F
        Photometric adaptation of Dole's microdetermination of free fatty acids.
        J Lipid Res. 1965; 6: 157
        • Frohman LA
        • Horton ES
        • Lebovitz HE
        Growth hormone releasing action of a pseudomonas endotoxin (Pyromen).
        Metabolism. 1967; 16: 57
        • Grenuth S
        • Frohman LA
        • Lebovitz HE
        A radioimmunological assay method for insulin using insulin — I125 and gel filtration.
        J Clin Endocrinol. 1965; 25: 1043
        • Jungas RL
        • Ball EG
        Studies on the metabolism of adipose tissue. XII. The effects of insulin and epinephrine on free fatty acid glycerol production in the presence and absence of glucose.
        Biochemistry. 1963; 2: 383
        • Fain JN
        • Kovacev VP
        • Scow RO
        Antilipolytic effect of insulin in isolated fat cells of the rat.
        Endocrinology. 1966; 78: 773
        • Pozefsky T
        • Felig P
        • Tobin J
        • Soeldner J
        • Cahill G
        Amino acid balance across tissues of the forearm in post absorptive man, effects of insulin at two dose levels.
        J Clin Invest. 1969; 48: 2273
        • Pruett EDR
        Plasma insulin concentrations during prolonged work at near maximal oxygen uptake.
        J Appl Physiol. 1970; 29: 155
        • Warren J
        • Felig P
        • Ahlborg G
        • Jorfeldt L
        Glucose metabolism during leg exercise in man.
        J Clin Invest. 1971; 50: 2715
        • Porte D
        • Williams RH
        Inhibition of insulin release by norepinephrine in man.
        Science. 1966; 152: 1248
        • Haggendal J
        • Hartley LH
        • Saltin B
        Arterial noradrenalin during exercise in relation to the relative work levels.
        Scand J Clin Lab Invest. 1970; 26: 337
        • Kneer P
        • Ball EG
        Studies on the metabolism of adipose tissue. XXI. An evaluation of the major pathways of pyruvate metabolism.
        J Biol Chem. 1968; 243: 2863
        • Madison LL
        • Mebane D
        • Unger RH
        • Lochner A
        The hypoglycemic action of ketones. II. Evidence for a stimulatory feedback of ketones on the pancreatic beta cells.
        J Clin Invest. 1964; 43: 408
        • Balasse E
        • Couturier E
        • Frankson JRM
        Influence of sodium B-hydroxybutyrate on glucose and free fatty acid metabolism in normal dogs.
        Diabetologia. 1967; 3: 488
        • Senior B
        • Loridun L
        Direct regulatory effect of ketones on lipolysis and on glucose concentrations in man.
        Nature. 1968; 219: 83
        • Balasse E
        • Ooms HA
        Changes in the concentration of glucose, free fatty acids, insulin and ketone bodies in the blood during sodium B-hydroxybutyrate infusions in man.
        Diabetologia. 1968; 4: 133
        • Fajans SS
        • Floyd Jr, JC
        • Knopf RF
        • Conn JW
        A comparison of leucine and acetoacetate-induced hypoglycemia in man.
        J Clin Invest. 1964; 43: 2003
        • Miller HI
        • Issekutz B
        • Paul P
        • Rodahl K
        Effect of lactic acid on plasma free fatty acids in pancreatectomized dogs.
        Am J Physiol. 1964; 207: 1226
        • Hawkins RA
        • Albert K
        • Houghton C
        • Williamson DH
        • Krebs HA
        The effect of aceto-acetate on plasma insulin concentration.
        Biochem J. 1971; 125: 541
        • Hellman D
        • Senior B
        • Goodman HM
        Anti-lipolytic effects of β-hydroxybutyrate.
        Metabolism. 1969; 18: 906
        • Vaughn M
        • Berger JE
        • Steinberg D
        Hormone-sensitive lipase and monoglyceride lipase activities in adipose tissue.
        J Biol Chem. 1964; 239: 401