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Abstract
Changes in high density lipoprotein (HDL) subfraction structure and composition were
analyzed during and after extracorporeal removal of apo B containing lipoproteins
in seven familial hypercholesterolemic (FH) patients. After the apheretic procedure,
carried out with dextran-sulfate-cellulose columns, the plasma levels of very low
density lipoproteins (VLDL), low density lipoproteins (LDL), and HDL decreased by
72%, 50%, and 19%, respectively. The free cholesterol to esterified cholesterol ratio
in plasma increased, with a 26% drop in the lecithin: cholesterol acyl transferase
(LCAT) activity. In the ensuing 24 hours, VLDL, HDL, and LCAT activity approached
the pretreatment levels. During this phase, possibly as a consequence of increased
cholesterol esterification and exchange of cholesteryl esters for triglycerides between
HDL and VLDL, HDL2a particles were detected in plasma. However, these metabolic changes did not result
in clearcut modifications in the HDL2-HDL3 subfraction distribution. These findings clearly demonstrate that rapid changes in
the plasma VLDL-LDL levels affect several processes involved in the HDL metabolism,
but confirm that the HDL system, in spite of a considerable plasticity, displays a
marked stability of the HDL2-HDL3 subfraction distribution.
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References
- Ultracentrifugal analysis of serum lipoproteins.Methods Biochem Anal. 1954; 1: 459-478
- Characterization of human high density lipoproteins by zonal ultracentrifugation.J Biol Chem. 1980; 255: 3178-3185
- Nondenaturing polyacrylamide gradient gel electrophoresis.Methods Enzymol. 1986; 128: 417-431
- High density lipoprotein metabolism.J Lipid Res. 1984; 25: 1017-1058
- Changes in the size and density of human high-density lipoprotein promoted by a plasma-conversion factor.Biochim Biophys Acta. 1986; 875: 429-438
- Liver lipase and high density lipoprotein. Lipoprotein changes after incubation of human serum with rat liver lipase.Biochim Biophs Acta. 1983; 751: 393-400
- Human serum lipoproteins and their interrelationships.Adv Biol Med Physics. 1967; 11: 109-158
- Regulation of human plasma lecithin: Cholesterol acyltransferase activity by lipoprotein acceptor cholesteryl ester content.J Biol Chem. 1981; 256: 2102-2104
- A cholesteryl ester transfer complex in human plasma.in: ed 9. Proc Natl Acad Sci USA. 77. 1980: 3327-3330
- Role of lipid transfers in the formation of a subpopulation of small high density lipoproteins.J Lipid Res. 1985; 26: 218-229
- Effects of postprandial lipemia on plasma cholesterol metabolism.J Clin Invest. 1985; 75: 874-882
- Selective removal of low density lipoprotein by plasmapheresis in familial hypercholesterolemia.Arteriosclerosis. 1985; 5: 613-622
- The LDL receptor defect in familial hypercholesterolemia.Med Clin North Am. 1982; 66: 335-362
- Heparin and the atherosclerotic process.Pharmacol Rev. 1984; 36: 91-109
- Dextran sulfate precipitation procedure for quantitation of high-density lipoprotein.Clin Chem. 1982; 28: 1379-1388
- The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum.J Clin Invest. 1955; 34: 1345-1355
- Effects of storage on the distribution of high density lipoprotein subfractions in human sera.J Lipid Res. 1985; 26: 1368-1373
- Enzymatische Bestimmung des GesamtCholesterins in Serum.Z Klin Chem Klin Biochem. 1974; 12: 403-407
- Quantitative determination of serum triglycerides by the use of enzymes.Clin Chem. 1973; 19: 476-482
- A new enzymatic method for determination of serum choline-containing phospholipids.Clin Chim Acta. 1977; 79: 93-96
- Protein measurement with the Folin phenol reagent.J Biol Chem. 1951; 193: 265-275
- Immunoturbidimetric assays of apolipoproteins A, AI, AII and B in serum.Clin Chem. 1986; 32: 957-961
- Determination of LCAT in human blood plasma.Scand J Clin Lab Invest. 1971; 27: 21-29
- Relationship of the phenotypic expression of the AI-Milano apoprotein with plasma lipid and lipoprotein patterns.Atherosclerosis. 1985; 58: 159-174
- Comparison of selectivity of LDL removal by double filtration and dextran-sulfate cellulose column plasmapheresis.Atherosclerosis. 1986; 60: 23-27
- Comparison of selectivity of LDL removal by double filtration and dextran-sulfate cellulose column plasmapheresis, and changes of subfractionated plasma lipoproteins after plasmapheresis in heterozygous familial hypercholesterolemia.Metabolism. 1987; 36: 419-425
- Influence of mevinolin on metabolism of low density lipoproteins in primary moderate hypercholesterolemia.J Lipid Res. 1985; 26: 1464-1475
- Effects of heparin infusion on plasma lipoproteins in subjects with lipoprotein lipase deficiency.FEBS Lett. 1982; 150: 255-259
- Synthetic substrates of lecithi: cholesterol acyltransferase.J Lipid Res. 1986; 27: 689-698
- Increases in the particle size of high-density lipoproteins induced by purified lecithin-cholesterol acyltransferase: Effect of low-density lipoproteins.Biochim Biophys Acta. 1986; 877: 406-414
- Role of triglyceride rich lipoproteins and hepatic lipase in determining the particle size and composition of high density lipoproteins.J Lipid Res. 1986; 27: 1265-1277
- Interconversion of high density lipoproteins during incubation of human plasma.Biochim Biophys Res Comm. 1981; 100: 391-399
- Direct evidence that reverse cholesterol transport is mediated by high density lipoprotein in the rabbit.Nature. 1985; 314: 109-111
Article info
Footnotes
☆Supported in part by the Consiglio Nazionale delle Ricerche of Italy, PF Ingegneria Genetica e Basi Molecolari delle Malattie Ereditarie.
Identification
Copyright
© 1988 Published by Elsevier Inc.
