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Abstract
Exogenous galanin has been shown to suppress insulin secretion as elicited by a number
of secretagogues such as glucose, arginine, tolbutamide, carbachol, and oral nutrients.
To achieve further insight into the influence of galanin on the endocrine pancreas,
we have investigated the effect of synthetic porcine galanin (a 200 ng bolus followed
by constant infusion at a concentration of 16.8 ng/mL for 16 to 24 minutes) on unstimulated
insulin, glucagon, and somatostatin release, as well as on the responses of these
hormones to 1 nmol/L vasoactive intestinal peptide (VIP), 1 nmol/L gastric inhibitory
peptide (GIP), 1 nmol/L 26 to 33 octapeptide form of cholecystokinin (8-CCK) or 10
nmol/L glucagon in the perfused rat pancreas. Galanin infusion reduced unstimulated
insulin secretion by 60% without modifying glucagon and somatostatin output. Galanin
also blocked insulin release elicited by VIP, GIP, and 8-CCK; it did not affect the
glucagon responses to VIP and GIP, or the somatostatin responses to VIP, GIP, and
8-CCK. Finally, galanin inhibited the insulin output, but not the somatostatin release
induced by glucagon. In conclusion, in the perfused rat pancreas, galanin appears
to behave as a general inhibitor of insulin secretion. Since this neuropeptide does
not modify glucagon or somatostatin release, a direct effect of galanin on the B-cell
seems plausible.
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Article info
Footnotes
☆Supported by Grant Nos. 86/1072 and 87/858 from the Fondo de Investigaciones Sanitarias de la Seguridad Social and Grant No. 0893/81 from the Comisión Asesora de Investigación Cientifica y Técnica, Ministerio de Educación y Ciencia, Spain.
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Copyright
© 1988 Published by Elsevier Inc.
