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Abstract
The effect of 48-hour starvation on glucose metabolism was studied in six non-diabetic,
normal weight men using a hyperinsulinemic (100 mU/min/m2) glucose clamp (3.5 mmol/L). The rate of glucose oxidation was calculated from measurements
of respiratory gas exchange, after allowing for the oxidation of ketones and of protein.
During the glucose clamp, the whole body glucose disposal rate decreased from 39.8
(SEM 4.6) μmol/kg/min in the fed state to 24.1 (2.1) μmol/kg/min in the starved state
(P < .01), consistent with insulin “resistance.” The glucose oxidation rate decreased
from 21.8 (1.3) to 3.9 (1.4) μmol/kg/min with starvation (P < .001), but the nonoxidative glucose disposal rate was unchanged (18.0[3.9] μmol/kg/min
normally fed, and 20.2[1.2] μmol/kg/min starved). With starvation, the rate of glucose
uptake in the forearm during the glucose clamp was reduced from 59.4 to 15.4 μmol/min/L
forearm (SE 5.6, P < .01, ANOVA). There was a significant net increase in thermogenesis during the glucose
clamp in the normally fed state (0.27 [0.08] kJ/min, P < .01, ANOVA), but not following starvation (0.11 [0.09] kJ/min, NS, ANOVA). Therefore,
starvation caused decreases in oxidative glucose disposal and in forearm glucose uptake;
despite the whole body nonoxidative disposal rate of glucose being unchanged, the
associated net thermogenic response was diminished.
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References
- The influence of nutrient administration on energy expenditure in man.Clin Nutr. 1986; 5: 181-186
- The effect of underfeeding on the physiological response to food ingestion in normal weight women.Br J Nutr. 1988; 60: 39-48
- Hormone-fuel interrelationships during fasting.J Clin Invest. 1966; 45: 1751-1769
- Laboratory procedures useful in diagnosis and treatment.in: Marble A White P Bradley RF Joslin's Diabetes Mellitus. ed 11. Lea & Febiger, Philadelphia, PA1971: 191-208
- Insulin action during acute starvation: evidence for selective insulin resistance in normal man.Metabolism. 1983; 32: 590-596
- The effect of insulin on the disposal of intravenous glucose. Results from indirect calorimetry and hepatic and femoral venous catheterization.Diabetes. 1981; 30: 1000-1007
- Glucose clamp technique: A method for quantifying insulin secretion and resistance.Am J Physiol. 1979; 237: E214-E223
- Effects of arterial versus venous sampling on analysis of glucose kinetics in man.J Appl Physiol. 1976; 41: 565-573
- The effect of graded doses of insulin on total glucose uptake, glucose oxidation, and glucose storage in man.Diabetes. 1982; 31: 957-963
- The quantitatively minor role of carbohydrate in oxidative metabolism by skeletal muscle in intact man in the basal state. Measurements of oxygen and glucose uptake and carbon dioxide and lactate production in the forearm.J Clin Invest. 1956; 35: 671-682
- Enzymic fluorometric continous-flow assays for blood glucose, lactate, pyruvate, alanine, glycerol and 3-hydroxy butyrate.Clin Chem. 1978; 34: 1724-1729
- An improved technique for extracting catecholamines from body fluids.J Neurosci Meth. 1985; 13: 239-248
- The measurement of volume changes in human limbs.J Physiol (Lond). 1953; 121: 1-27
- An automated method for the measurement of oxygen consumption and carbon dioxide excretion in man.Clin Phys Physiol Meas. 1985; 6: 349-355
- New methods for calculating metabolic rate with special reference to protein metabolism.J Physiol (Lond). 1949; 109: 1-9
- Estimation of energy expenditure, net carbohydrate utilization, and net fat oxidation and synthesis by indirect calorimetry: evaluation of errors with special reference to the detailed composition of fuels.Am J Clin Nutr. 1988; 47: 608-628
- Assessment of energy expenditure and fuel utilization in man.Annu Rev Nutr. 1987; 7: 187-208
- The influence of a low carbohydrate diet on forearm metabolism in man.Diabetes. 1973; 22: 145-159
- Mechanism of glucose intolerance during fasting: differences between lean and obese subjects.Metabolism. 1977; 26: 1147-1153
- Impaired glucose metabolism in surgical patients improved by intravenous nutrition: Assessment by the euglycemic-hyperinsulinemic clamp.Metabolism. 1988; 37: 505-509
- Influence of a 60-hour fast on insulin-mediated splanchnic and peripheral glucose metabolism in humans.J Clin Invest. 1985; 76: 87-92
- Fluctuations in the affinity and concentration of insulin receptors on circulating monocytes of obese patients.J Clin Invest. 1976; 58: 1123-1135
- Insulin binding to monocytes and insulin action in human obesity, starvation and refeeding.J Clin Invest. 1978; 62: 204-213
- The effects of acute and chronic starvation on insulin binding to isolated human adipocytes.J Clin Endocrinol Metab. 1979; 48: 836-842
- Fasting decreases rates on noninsulin-mediated glucose uptake in man.J Clin Endocrinol Metab. 1988; 67: 532-540
- Dose response characteristics for effects of insulin on production and utilization of glucose in man.Am J Physiol. 1981; 240: E630-E639
- The glucose-fatty acid cycle. Its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus.Lancet. 1963; 1: 785-789
- Effect of fatty acids on glucose production and utilization in man.J Clin Invest. 1983; 72: 1734-1747
- Inhibition of muscular glucose uptake by lipid infusion in man.Clin Nutr. 1986; 5: 187-192
- Effect of insulin on carbohydrate metabolism and on potassium in the forearm of man.J Clin Invest. 1962; 41: 108-115
- Carbohydrate storage in man: Speculations and some quantitative considerations.Metabolism. 1978; 27: 1853-1865
- Thermic effect of glucose in man: Obligatory and facultative thermogenesis.J Clin Invest. 1984; 74: 1572-1580
- The biochemistry of energy expenditure.in: Bray GA Recent Advances in Obesity Research II. Newman, London, England1978: 211-228
- The effect of a 48 h fast on the thermoregulatory responses to graded cooling in man.Clin Sci. 1984; 67: 445-452
- The energy cost of triglyceride-fatty acid recycling in non-obese subjects after an overnight fast and four days of starvation.Metabolism. 1987; 36: 251-255
- Substrate cycles: Their role in control of metabolism with specific references to the liver.Diabetes Metab Rev. 1987; 3: 295-305
- Effect of insulin and glucose infusions on sympathetic nervous system activity in normal man.Diabetes. 1981; 30: 219-225
- Noradrenaline turnover during under- and overeating in normal weight subjects.Metabolism. 1982; 31: 896-899
- Dissociation of sympathetic nervous system and adrenal medullary responses.Am J Physiol. 1984; 247: E35-E40
- Glycemic thresholds for activation of glucose counterregulatory systems are higher than the threshold for symptoms.J Clin Invest. 1987; 79: 777-781
- Epinephrine sensitivity with respect to metabolic rate and other variables in women.Am J Physiol. 1983; 245: E431-E442
- Facultative thermogenesis induced by carbohydrate: A skeletal muscle component mediated by epinephrine.Am J Physiol. 1986; 250: E226-E229
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Footnotes
☆Supported in part by a Project Grant from the Wellcome Trust.
Identification
Copyright
© 1990 Published by Elsevier Inc.
