Advertisement

Exercise training prevents maturation-induced decreases in insulin receptor substrate-1 and phosphatidylinositol 3-kinase in rat skeletal muscle

  • Masaru Nagasaki
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
  • Naoya Nakai
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
  • Yoshiharu Oshida
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
  • Zhihao Li
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
  • Ming Xu
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
  • Mariko Obayashi
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
  • Taro Murakami
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
  • Atsushi Yoshimura
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
  • Noriaki Fujitsuka
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
  • Yoshiharu Shimomura
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
  • Yuzo Sato
    Affiliations
    From the Department of Sports Medicine, Graduate School of Medicine, and Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya; and Department of Bioscience, Nagoya Institute of Technology, Nagoya, Japan.
    Search for articles by this author
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      We have previously reported that exercise training prevents a maturation-induced decrease in insulin sensitivity and suggested that an improvement of insulin sensitivity by exercise training was attributable, in part, to an increase in insulin-sensitive GLUT-4 on the skeletal muscle plasma membrane. In this study, we examined the effects of maturation and exercise training on the gene expression and protein content of the components of post-insulin receptor signal transduction in rat skeletal muscle. Rats aged 3 weeks were sedentary or trained by voluntary running through 4 or 27 weeks of age, and then the rats in both the sedentary and trained groups were killed and the gastrocnemius muscle was immediately removed for analysis of mRNA and protein content. The concentration of mRNA and protein for insulin receptor substrate-1 (IRS-1) in sedentary rats significantly decreased with maturation (49% and 63%, respectively, at age 27 weeks v age 4 weeks), but in trained rats they did not decrease with maturation. Although the level of phosphatidylinositol 3-kinase (PI 3-kinase) mRNA in sedentary rats was not altered with maturation, PI 3-kinase protein in sedentary rats significantly decreased with maturation (73% at 27 weeks v 4 weeks). However, PI 3-kinase protein in trained rats did not decrease with maturation. These results suggest that the prevention of maturation-induced decreases in the protein content of IRS-1 and PI 3-kinase is involved in the mechanisms responsible for the improvement of insulin sensitivity by exercise training, and exercise training may affect transcriptional regulation of the IRS-1 gene and posttranscriptional regulation of PI 3-kinase expression.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Metabolism - Clinical and Experimental
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect