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A close relationship between elevated plasma free fatty acid (FFA) levels and insulin
resistance is commonly reported in obese subjects. The aim of the present study was
to evaluate the role of intramuscular triglyceride (mTG) and FFA levels in insulin
sensitivity in 30 nondiabetic normal-weight or obese subjects (18 with body mass index
[BMI] = 21.8 ± 3.3 kg/m2 and 12 with BMI = 34.6 ± 2.7 kg/m2) who underwent minor abdominal surgery. Body composition was estimated by isotopic
dilution, substrate oxidation by indirect calorimetry, and whole-body glucose uptake
by euglycemic-hyperinsulinemic clamp (EHC). Glucose uptake (M) value negatively correlated
with the MTG level (R2 = −.56, P < .0001), which was increased in obese different in the 2 groups: an increased concentration
of saturated fat was present in obese patients (unsaturated to saturated ratio, 1.89
± 0.40 v 2.19 ± 0.07, P < .0001). Stepwise linear regression analysis of total mTGs and palmitic and oleic
fractions on the M value showed that only TGs and plamitic acid were significantly
related to glucose uptake (R2 = .66, P < .0001). Furthermore, among the other anthropometric variables, only the BMI was
significantly correlated with MTGs (R2 = .71, P < .0001). In conclusion, not only the MTG concentration but also the FFA pattern
seems to affect insulin-mediated glucose uptake. A pivotal role might be played by
a high saturated fatty acid content in the TGs.
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Article info
Publication history
Accepted:
July 15,
1999
Received:
April 12,
1999
Identification
Copyright
© 2000 W.B. Saunders Company. Published by Elsevier Inc.
