Abstract
Objective
The objective was to evaluate the protective effect of ellagic acid against experimentally
induced cardiac arrhythmias, hypertrophy and its association with altered lipid metabolism
during myocardial infarction in rats.
Methods
Rats were treated with ellagic acid (7.5 and 15 mg/kg) orally for a period of 10 days. After 10 days of pretreatment, isoproterenol (100 mg/kg) was injected subcutaneously at an interval of 24 h for 2 days to induce myocardial infarction. On the 12th day, the cardiac rhythm was observed.
The rats were sacrificed and the heart was isolated from each rat. Ventricular hypertrophy
and myocardial necrotic scores were analysed in the myocardium. Lipid peroxidation
products in the plasma were analysed. Changes in the lipid profile were measured using
the plasma and heart tissue homogenates of normal and experimental rats.
Results
Isoproterenol-induced rats showed arrhythmias, hypertrophy and increased levels of
myoglobin, creatine kinase-MB, lipid peroxidation products compared to the normal
control rats. Ventricular hypertrophy and increased myocardial necrotic scores were
observed in isoproterenol-induced rats. Oral pretreatment with ellagic acid restored
pathological arrhythmias, ventricular hypertrophy, lipid peroxidation, altered lipid
profile and myocardial necrosis in the isoproterenol-induced myocardial infarcted
rats.
Conclusions
Oral pretreatment with ellagic acid was safe and effective in cardio protection against
ISO-induced arrhythmias, hypertrophy and myocardial necrosis. Anti lipid peroxidation
property and anti hyperlipidaemic activity through 3-hydroxy-3 methyl glutaryl CoA
reductase inhibition by ellagic acid may be the reasons for the beneficial action
of ellagic acid against experimentally induced myocardial infarction.
Abbreviations:
MI (myocardial infarction), ISO (isoproterenol), PVC (premature ventricular contraction), VF (ventricular fibrillation), CK-MB (creatine kinase-MB), HDL (high density lipoprotein), LDL (low density lipoprotein), VLDL (very low density lipoprotein), FFA (free fatty acid), HMG-CoA reductase (3-hydroxy-3 methyl glutaryl CoA reductase), NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells.)Keywords
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Article info
Publication history
Published online: October 10, 2012
Accepted:
June 6,
2012
Received:
June 22,
2011
Identification
Copyright
© 2013 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
