Abstract
Objective
Adiponectin and C1q have similar sequences, exist abundantly in blood, and are produced
by adipose tissues. The aim of this study was to examine whether adiponectin and C1q
form protein-complex in blood and to know the clinical significance of the C1q–adiponectin
(C1q–APN) complex in serum.
Methods
The direct interaction between adiponectin and C1q was investigated by far western
blotting and co-immunoprecipitation. The relationship between serum C1q–APN and various
clinical features was analyzed in 329 Japanese men who underwent health check-up,
including measurements of visceral (VFA) and subcutaneous fat area (SFA) by computed
tomography (Victor-J study).
Results
Adiponectin bound to C1q in vitro and C1q–APN complex existed in human blood. C1q–APN complexes were identified in
high- and middle-molecular weight forms of adiponectin in human serum by gel-filtration
chromatography. Stepwise multiple regression analysis identified body mass index,
VFA and SFA as significant determinants of serum C1q–APN level. Serum C1q–APN/Total-APN
ratio correlated positively with cardiovascular risk factor accumulation in subjects
with VFA ≥100 cm2.
Conclusions
These results indicate that high- and middle-molecular forms of adiponectin partly
consist of adiponectin-complex with other proteins including C1q and that the blood
C1q–APN/Total-APN ratio may serve as a biomarker of the metabolic syndrome in general
male subjects.
Abbreviations:
C1q–APN (C1q-binding adiponectin), FWB (far western blotting), HMW (high-molecular weight), LMW (low-molecular weight), MMW (middle-molecular weight), HMW-APN (high molecular weight-adiponectin), rhAPN (recombinant human adiponectin), Total-APN (total-adiponectin)Keywords
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Article info
Publication history
Published online: July 23, 2012
Accepted:
June 18,
2012
Received:
May 8,
2012
Footnotes
Clinical Trial Registration Number: UMIN 000004318. https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr.cgi?function=brows&action=brows&type=summary&recptno=R000005173&language=E.
Identification
Copyright
© 2013 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
