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Abstract
To study the effect of a moderate increase in insulin secretion produced by an increased
daily protein intake on dehydroepiandrosterone sulfate (DHEAS), a balanced randomized
crossover trial consisting of three strictly controlled dietary regimens was performed
in six healthy male volunteers. The basic diet (B) contained 50 g protein/d; diets
P and M (also basic diets) were enriched with either 32 g protein/d (P) or 10 mmol
l-methionine/d (M). Methionine was given (as a specific nonprotein source of endogenously
derived sulfate) to control for possible confounding effects on DHEAS due to an increased
sulfate supply. At the end of each 4-day diet period, blood and 24-hour urine samples
were collected. Fasting plasma levels of testosterone, cortisol, insulin-like growth
factor-I (IGF-I), and insulin, as well as urinary output of total (hot acid-cleaved)
testosterone conjugates and 3α-androstanediol glucuronide, did not show significant
changes in response to dietary manipulations. Endogenous sulfate availability (as
reflected by renal sulfate output per 24 hours) approximately doubled with diets P
and M. However, plasma levels (6.3 ± 1.5, 6.8 ± 1.8, and 6.9 ± 2.1 μmol/L for B, P,
and M, respectively) and urinary excretion (8.8 ± 9.8, 9.4 ± 11.2, 8.0 ± 8.3 gmmol/d)
of DHEAS remained unaffected. Considering the clear increments (P < .01) in urinary C-peptide excretion with diet P (20.4 ± 10.3 nmol/d) versus diets
B and M (12.6 ± 5.1 and 13.2 ± 3.6 nmol/d), respectively, our results suggest that
a moderately strong diet-induced increase in daily insulin secretion does not alter
urinary and plasma levels of DHEAS.
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Article info
Publication history
Accepted:
June 18,
1996
Received:
November 25,
1995
Footnotes
☆Supported by the Ministerium für Wissenschaft and Forschung des Landes Nordrhein-Westfalen and the Bundesministerium für Gesundheit.
☆☆Presented in part at the International Symposium on DHEA Transformation into Androgens and Estrogens in Target Tissues: Intracrinology, Québec City, Canada, September 13–15, 1995, and at the 40th Symposium of the German Society of Endocrinology, Marburg, Germany, February 29–March 2, 1996.
Identification
Copyright
© 1996 Published by Elsevier Inc.
